Senescence Cells Histochemical Staining Kit
SIGMA/CS0030 - sufficient for 100 tests
Synonym: Senescent cells IHC kit; cellular β-galactosidase probe
Product Type: Chemical
Detection of senescent cells. Primary Human Foreskin Fibroblasts (HFF) at early and late passages (5 and 28 passages, respectively) were stained using the Senescent Cell Staining Kit (Prod. No. CS0030). The HFF cells at passage 28 show a blue staining indicating that these cells are senescent, whereas at passage 5, senescent cells are absent.
| detection method | colorimetric |
| packaging | pkg of 1 kit |
| Quality Level | 200  |
| shipped in | dry ice |
| storage condition | dry at room temperature |
| storage temp. | −20°C |
| usage | sufficient for 100 tests |
| Application: | Senescence Cells Histochemical Staining Kit has been used for: • senescence-associated β-galactosidase assay • to test for senescence in human pancreatic cancer cells • adipose-derived stem cells (ADSCs) • cord blood mesenchymal stromal/stem cells (CBMSC) |
| Biochem/physiol Actions: | Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells. |
| General description: | The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for β-galactosidase activity at pH 6. β-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells. Cellular senescence is a progression of events, whereby cells move from an actively dividing to a non-dividing stage. The cellular senescence process is associated with aging. The decrease in cell division is virtually irreversible and complete. In conjunction with the loss of the ability to divide, changes occur in the morphology, shape, and physical appearance of the cells, and their pattern of gene expression. At the end of the process cell death usually occurs, although the cells may remain viable for a long time. |
| Legal Information: | Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728. |
| Symbol |    GHS05,GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H302 + H312 - H314 - H317 - H331 - H334 - H335 - H341 - H350 - H412 |
| Precautionary statements | P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | C |
| Risk Statements | 20/21/22-34-40-42/43 |
| Safety Statements | 23-26-36/37/39-45 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |
| Components | X-gal solution 4 mL; Staining Solution, 10X 15 mL; Fixation Buffer 10x 15 mL; Reagent B 1.5 mL; Reagent C 1.5 mL; Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL |