Glutathione Assay Kit
SIGMA/CS0260 - sufficient for 700 assays
Synonym: GSH Quantification Test
Product Type: Chemical
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Glutathione structure.
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| usage | sufficient for 700 assays |
| Application: | Glutathione Assay Kit has been used to measure total glutathione concentration in glutathione assay. It also has been used to determine different oxidative stress parameters in rats. |
| Biochem/physiol Actions: | Glutathione (GSH) functions as a cofactor for several antioxidant enzymes. It is involved in the regeneration of vitamins C and E. It modulates cellular proliferation and apoptosis. GSH is crucial for mitochondrial functioning and the maintenance of mitochondrial DNA (mtDNA). Mutations in this gene are associated with Alzheimer′s, Parkinson′s and Huntington′s diseases. It is also linked to cystic fibrosis, immune diseases and cardiovascular diseases. |
| Biochem/physiol Actions: | Reduced glutathione (GSH), a tripeptide (g-glutamyl-cysteinylglyc |
| Features and Benefits: | • A quick and simple method • The kit contains all the reagents required for a colorimetric assay of total glutathione (oxidised + reduced GSSG + GSH) • The kit includes a deproteination reagent for biological samples |
| General description: | The kit provides all reagents for simple and quick assay to measure the level of total glutathione (GSSG + GSH) in a cell and tissue extracts and in red blood cells or plasma. The sample is first deproteinized with the 5% 5-sulfosalicylic acid solution. Glutathione content of the sample is then assayed using a kinetic assay in which catalytic amounts of glutathione cause a continuous reduction of 5,5′-dithiobis-(2-nitrobe |
| Symbol |   GHS05,GHS08 |
| Signal word | Danger |
| Hazard statements | H314 - H334 |
| Precautionary statements | P261 - P280 - P284 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26 |
| WGK Germany | WGK 3 |
| Flash Point(F) | 188.6 °F |
| Flash Point(C) | 87 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161503 |
| Components | Assay Buffer 5x 30 mL; DTNB 8 mg; DMSO 7.5 mL; Glutathine Reductase 20 U; L-Glutathione Reduced 300 μg; NADPH 25 mg; 5-Sulfosalicylic Acid 2.5 g |