Mitochondria Staining Kit
SIGMA/CS0390 - 1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)
Synonym: JC-1 dye
Product Type: Chemical
The principle of all the following detection methods is a comparison between the red fluorescence of the aggregated JC-1, representing intact mitochondria, to the green fluorescence of the monomeric JC-1, which represents disrupted mitochondria. Staurosporine induce apoptosis and reduce the accumulation of the JC-1 dye in the mitochondria. Valinomycin prevent JC-1 aggregation.
| application(s) | cell analysis detection |
| detection method | fluorometric |
| fluorescence | λex 490 nm; λem 530 nm (green) (JC-1 monomers) |
| λex 525 nm; λem 590 nm (red) (JC-1 aggregates) | |
| packaging | pkg of 1 kit |
| Quality Level | 200  |
| shipped in | wet ice |
| storage condition | dry at room temperature |
| storage temp. | 2-8°C |
| technique(s) | flow cytometry: suitable |
| protein staining: suitable | |
| usage | kit sufficient for 200 tests (of 1 mL cell suspensions) |
| kit sufficient for 40 tests (of 5 mL cell suspensions) |
| Application: | The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1. |
| Features and Benefits: | Kit offers: • A fast, simple, and convenient method for staining and assaying mitochondria intactness • Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential • Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation • The shift in fluorescence is clearly detectable in both green and red channels • Suitable for adherent cells as well as cells in suspension • Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells • The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry |
| General description: | In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |
| Components | DMSO 1 mL; JC Staining Buffer 5X 120 mL; JC-1 1 mg; Valinomycin Ready Made .1 mL |