Isolated Mitochondria Staining Kit
SIGMA/CS0760 - 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)
EC Number: 217-896-6
Product Type: Chemical
RFU – Relative Fluorescence units. Mitochondria were isolated from CHO cells using the Cell Mitochondria Isolation Kit (Catalog Number MITOISO2) and stained in a multiwell plate using the Isolated Mitochondria Staining Kit. The upper line represents the JC-1 dye uptake of an intact mitochondrial sample. The lower line represents the dye uptake of the valinomycin treated mitochondrial control sample.
| application(s) | cell analysis detection |
| detection method | fluorometric |
| fluorescence | λex 490 nm; λem 590 nm (JC-1 dye) |
| packaging | pkg of 1 kit |
| Quality Level | 200  |
| shipped in | dry ice |
| storage condition | dry at room temperature |
| storage temp. | −20°C |
| technique(s) | flow cytometry: suitable |
| protein staining: suitable | |
| usage | kit sufficient for 1000 reactions (using 96 multiwell plates) |
| kit sufficient for 50 reactions (in a 2 mL cuvette) |
| Analysis Note: | The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential. |
| Application: | Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential. |
| Biochem/physiol Actions: | The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope. |
| Features and Benefits: | • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation |
| Symbol |  GHS06 |
| Signal word | Danger |
| Hazard statements | H300 + H310 |
| Precautionary statements | P264 - P280 - P301 + P310 - P302 + P350 - P310 |
| Hazard Codes | T+ |
| Risk Statements | 27/28 |
| Safety Statements | 28-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352207 |
| Components | DMSO 1 mL; JC-1 Assay Buffer 5X 25 mL; JC-1 Stain 25 μg; Valinomycin Ready Made 100 μL |