Antioxidant Assay Kit
SIGMA/CS0790 - sufficient for 200 tests
Synonym: Oxidative Stress Detection Kit
Product Type: Chemical
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The principle of the antioxidant assay is formation of a ferryl myoglobin radical from metmyoglobin and hydrogen peroxide, which oxidizes the ABTS (2,2′-azino-bis(3 ethylbenzthiazoline-6-sulfonic acid) to produce a radical cation, ABTS, a soluble chromogen
that is green in color and can be determined
spectrophotometrically at 405 nm.
that is green in color and can be determined
spectrophotometrically at 405 nm.
Trolox standard clibration curve
| detection method | colorimetric |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| usage | sufficient for 200 tests |
| Application: | Antioxidant assay kit provides all of the reagents required for an efficient measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates. It has also been used to measure the radical cation (2,2′-azino-di-[3-ethylbenzthiazoline sulphonate]) (ABTS+) scavenging capacity. |
| Application: | It was tested on A431 and CHO cell lysates; rat brain, liver, and kidney lysates; human plasma, serum, urine, and saliva. |
| Application: | Suitable for the measurement of the total antioxidant capacity of plasma, serum, urine, saliva, cells, and tissue lysates |
| Biochem/physiol Actions: | The principle of the antioxidant assay is formation of a ferryl myoglobin radical from metmyoglobin and hydrogen peroxide, which oxidizes the ABTS (2,2¢-azino-bis(3-ethylbe |
| General description: | Free radicals or reactive oxygen species (ROS) are produced during biochemical redox reactions as part of normal physiological cell metabolism (protection from infectious organisms) and as a response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and g-radiation. Once formed, ROS attack cellular components causing damage to lipids, proteins, and DNA, which can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders. |
| General description: | In the presence of antioxidants the radical cation is suppressed to an extent dependent on the activity of the antioxidant and the color intensity is decreased proportionally. Trolox, a water-soluble vitamin E analogue, serves as a standard or a control antioxidant. |
| Preparation Note: | Myoglobin Solutions are to be stored at -20 °C after reconstitution. Trolox Working Solution is to be stored at -20 °C after reconstitution. ABTS Substrate Solution is to be stored at 4 °C for 2 weeks or at -20 °C for 1 month. |
| Preparation Note: | Reagents are to be prepared with ultrapure water. |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H302 - H315 - H318 - H335 - H412 |
| Precautionary statements | P261 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-37/38-41 |
| Safety Statements | 26-39 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161503 |
| Components | Assay Buffer 10X 30 mL; Stop Solution 20 mL; Hydrogen Peroxide, 3% solution 1 mL; Myoglobin from horse heart 2 x 1 mg; (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox®) 2 x 1 mg; ABTS, 10 mg tablets 4 tab; Phosphate-Citrate Buffer, pH 5 4 tab |