Chitinase Assay Kit
SIGMA/CS0980 - sufficient for 100 multiwell tests
Synonym: Chitinase Activity Detection Kit
Product Type: Chemical
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Unit Definition One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C.
Chitinases have been detected in many organisms, including bacteria, fungi, plants, invertebrates and vertebrates. Chitinases are broadly classified as endo- and exochitinases. The endochitinase activity is defined as the random cleavage at internal points in the chitin chain. The exochitinase activity is defined as the progressive action starting at the non reducing end of chitin with the release of chitobiose or N-acetylglucosamine units. Chitobiosidase and N-acetyl-b-glucosaminidase are considered exochitinases. The combination of endo- and exochitinases results in a synergistic increase in the chitinolytic activity.
| absorbance ratio | 405 nm (Absorbance) |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| usage | sufficient for 100 multiwell tests |
| Analysis Note: | The kit assay is based on the enzymatic hydrolysis of chitinase substrates. This hydrolysis releases p-nitrophenol (4-nitrophenol), which upon ionization in basic pH can be measured colorimetrically at 405 nm. |
| Application: | Chitinase Assay Kit has been used in screening chitinase activity of recombinant chitinase from yeast, Metschnikowia fructicola, bacterial endophyte 3A12 and Clostridium difficile spores. The kit provides all the reagents required for efficient detection of chitinase activity in fungal and bacterial growth media, macrophage lysates, and purified enzyme preparations. In addition, the kit provides three different substrates for the detection of the various types of the chitinolytic activity: • 4-Nitrophenyl N,N′-diacetyl-β- • 4-Nitrophenyl N-acetyl-β- 4-Nitrophenyl β- |
| Biochem/physiol Actions: | Chitinase catalyzes the hydrolytic cleavage of the β-1→4-glycoside bond present in biopolymers of N-acetylglucosamine, primarily in chitin. Chitinases are widely distributed in living organisms and are found in fungi, bacteria, parasites, plants, and animals. They are classified in families based on amino acid sequence similarities. The chitinolytic enzymes are also categorized based on their enzymatic action on chitin substrates. Endochitinases are defined as the enzymes catalyzing the random cleavage at internal points in the chitin chain. Exochitinases catalyze the progressive release of acetylchitobiose or N-acetylglucosamine from the non-reducing end of chitin, and are referred to as chitobiosidase and β-N-acetylglucosaminidase Chitinases perform different functions in different organisms. In bacteria, they are mainly involved in nutritional processes. In yeast and various fungi, these enzymes participate in morphogenesis. In animals and plants, chitinases primarily play a role in the defense of the organism against pathogen attack. |
| General description: | The Chitinase Assay Kit is based on the enzymatic hydrolysis of chitinase substrates. The hydrolysis releases p-nitrophenol, which can be measured colormetrically at 405 nm. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161503 |
| Components | Assay Buffer 20 mL; 4-Nitrophenyl N-acetyl-β-D-glucosaminide 10 mg; 4-Nitrophenyl N,N′-diacetyl-β-D-chitobioside 5 mg; 4-Nitrophenyl β-D-N,N′N′′-triacetylchitotriose 1 mg; Chitinase from Trichoderma viride 1 mg; p-Nitrophenol Solution, 10 mM 1 mL; Sodium Carbonate 1 g |