Superoxide Anion Assay Kit
SIGMA/CS1000 - sufficient for 100 assays (96 well plates)
Synonym: Superoxide Detection Kit
Product Type: Chemical
Multiple enzymatic scavengers are utilized by the cell to limit damage from reactive oxygen species. These scavengers include members of the superoxide dismutase (SOD) family, catalase, and glutathione peroxidase.
Superoxide Anion Production by U937 cells. Purple - approapiret test. Green - Inhibition with SOD; In the presence of SOD the superoxide anion produced is immediately converted to hydrogen peroxide and therefore, there is no enhancement in the luminescence intensity. Blue - no enhancer - enhancer role is to amplifies the chemiluminescent signal. Deep Blue - no PMA - PMA is required in order to stimulate U937 cells to produce superoxide anions.
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| usage | sufficient for 100 assays (96 well plates) |
| Analysis Note: | The kit method is based on the oxidation of luminol by superoxide anions resulting in the formation of chemiluminescence light. This method utilizes a specific, non-toxic enhancer that amplifies the chemiluminescent signal. |
| Application: | The Superoxide Anion assay kit provides a useful qualitative cell-based assay for the measurement of O2− anion status in cells. The kit can be used to test changes in superoxide anion levels following oxidative stress directly in whole cells. In addition, the kit can be also used for the detection of superoxide dismutase (SOD) activity. This activity was tested on erythrocytes, leucocytes, and plasma. |
| Biochem/physiol Actions: | The superoxide anion (O2-) is a short-lived radical generated by the addition of an electron to oxygen. It is formed in response to environmental factors such as UV light, cigarette smoke, environmental pollutants, and γ-radiation, or by oxidases like xanthine oxidase or NADPH oxidase. Once formed, O2- attacks cellular components causing damage to lipids, proteins, and DNA. This can initiate numerous diseases, including cancer, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system disorders. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P305 + P351 + P338 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161503 |
| Components | Assay Buffer 25 mL; Assay Medium 20 mL; Enhancer Solution, 40 mM 500 μL; Luminol Solution 500 μL; Xanthine Solution 500 μL; Xanthine Oxidase .5 U; Superoxide Dismutase from bovine liver, 4 units/μL 400 U |