Cytochrome c Reductase (NADPH) Assay Kit
SIGMA/CY0100 - 1 kit sufficient for 100 tests, determining cytochrome c reductase activity
Product Type: Chemical
Cytochrome P450 protein complex: CYP450, cytochrome b5 and NADPH reductase
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| usage | kit sufficient for 100 tests |
| Analysis Note: | The Cytochrome C Reductase Assay Kit contains all the reagents required for a fast and convenient determination of NADPH-Cytochrome C Reductase activity in cell and tissue extracts and in purified microsomes. It has been tested on samples prepared from various species of mammalian tissues such as liver, kidney, brain, spleen, and heart muscle, as well as on lysates from cell lines such as HeLa, HepG2, and Jurkat. In addition, it has been tested on samples prepared from the yeast strains P. pastoris and S. cerevisiae. |
| Biochem/physiol Actions: | Eukaroytic NADPH-cytochrome c reductase (NADPH-cytochrome P450 reductase, EC1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which are the cytochrome P450 family of enzymes, responsible for xenobiotic detoxification. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as one of the biomarkers for ecological pollution and dietary lipid uptake. |
| Features and Benefits: | • Detection of endoplasmic reticulum (ER) during isolation and subcellular fractionation by density gradient separation • Colorimetric measurement of NADPH-Cytochrome C Reductase activity in various sources after intoxication by drugs or challenge by xenobiotics • Analyze enrichment and function of ER |
| Hazard Codes | Xn,N |
| Risk Statements | 20/21/22-51/53 |
| Safety Statements | 36/37-61 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161503 |
| Components | Assay Buffer 100 mL; Enzyme Dilution Buffer 25 mL; Cytochrome c 50 mg; β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mg; Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL; Cytochrome c Oxidase Inhibitor Solution 1 mL |