Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham
SIGMA/D0547 - With L-glutamine and trace elements, without HEPES and sodium bicarbonate, powder, suitable for cell culture
Synonym: DME/F-12, 1:1 mixture
MDL Number: MFCD00217417
Product Type: Chemical
| components | NaHCO3: no sodium pyruvate: 0.11 g/L HEPES: no phenol red: yes glucose: 3.15 g/L L-glutamine: yes |
| form | powder |
| Quality Level | 500  |
| shipped in | ambient |
| storage temp. | 2-8°C |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used: • to culture cerebral cortices from mice embryos • as a component of the human corneal epithelium (HCE) media for culturing human limbal explants • to culture human corneal epithelial cell lines |
| General description: | Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is a 1:1 mixture. |
| Other Notes: | Trace elements include ammonium molybdate, ammonium metavanadate, stannous chloride, sodium selenite, magnesium sulfate, nickel chloride, and sodium metasilicate |
| Preparation Note: | Formulated to contain 12.0 grams of powder per liter of medium. |
| Preparation Note: | Supplement with 1.2 g/L sodium bicarbonate |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P264 - P280 - P280 - P332 + P313 - P337 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |