3,5-Dinitrosalicylic acid
SIGMA/D0550 - used in colorimetric determination of reducing sugars
Synonym: 3,5-
CAS Number: 609-99-4
Empirical Formula (Hill Notation): C7H4N2O7
Molecular Weight: 228.12
EC Number: 210-204-3
MDL Number: MFCD00007104
Linear Formula: (O2N)2C6H2-2-(OH)CO2H
Product Type: Chemical
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥98% (HPLC and titration) |
| form | powder |
| InChI | 1S/C7H4N2O7/c10-6-4(7(11) |
| InChI key | LWFUFLREGJMOIZ-UHFFFAOYSA |
| mp | 168-172 °C (lit.) |
| Quality Level | 200 ![]() |
| SMILES string | OC(=O)c1cc(cc(c1O)[N+]([O |
| solubility | H2O: 50 mg/mL |
| storage temp. | room temp |
| Application: | 3,5-Dinitrosalicylic acid (DNS) has been used to stop the reaction in in vitro α-amylase inhibition study. It has also been used for the quantitation of enzymatically released reducing sugars. |
| Biochem/physiol Actions: | 3,5-Dinitrosalicylic acid (DNS) is used in colorimetric determination of reducing sugars and to analyze glycosidase (glycoside hydrolase) activity by quantitation of enzymatically released reducing sugar. The dinitrosalicylic acid method has been compared to the Nelson-Somogi colorimetric method. |
| Packaging: | 10, 25, 100, 500 g in glass bottle |
| Symbol | ![]() GHS02,GHS07 |
| Signal word | Danger |
| Hazard statements | H228 - H302 |
| Precautionary statements | P210 - P240 - P241 - P264 - P270 - P301 + P312 |
| Hazard Codes | Xn |
| Risk Statements | 22-37/38 |
| Safety Statements | 22-24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC and titration) |
| mp | 168-172 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |



