DNA Gyrase from Escherichia coli
SIGMA/D0690 - aqueous glycerol solution
MDL Number: MFCD00211877
Product Type: Chemical
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| application(s) | cell analysis |
| biological source | Escherichia coli |
| concentration | ≥2 unit/μL |
| form | aqueous glycerol solution |
| mol wt | ~374 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −70°C |
| technique(s) | cell based assay: suitable |
| UniProt accession no. | P0AES4  |
| P0AES6 |
| Application: | DNA gyrase from Escherichia coli has been used in a study to investigate a comparative proteomic approach to better define Deinococcus nucleoid specificities. DNA gyrase from Escherichia coli has also been used in a study to investigate the role of the DnaK-ClpB bichaperone system in DNA gyrase reactivation. |
| Biochem/physiol Actions: | Can be used to supercoil plasmids. |
| Biochem/physiol Actions: | DNA gyrase is supplied as an A2B2 holoenzyme. The molecular mass of subunit A is 97 kDa and that of subunit B is 90 kDa. It catalyzes the ATP-dependent introduction of negative supercoils into relaxed DNA. DNA gyrase has been successfully converted into a type II topoisomerase by mutagenesis experiments. |
| Other Notes: | One unit of gyrase activity will supercoil 0.5 micrograms of pBR-322 DNA in 30 minutes at 37 °C. |
| Other Notes: | Solution in 50% Glycerol containing Tris buffer, DTT and EDTA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 5.99.1.3 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |