Anti-DHFR, C-terminal antibody produced in rabbit
SIGMA/D0942 - 1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Dihydrofolate reductase
MDL Number: MFCD05665211
Product Type: Chemical
Immunoblotting
Western blot analysis of a DHFR titration: Decreasing amount of recombinant DHFR were loaded onto lanes of a mini gel separated on SDS-PAGE and blotted with Rabbit Anti-DHFR (C-Terminal) (Cat. No. D0942). Protein loaded per lane ranged from 12.5−200 ng.
Western blot analysis of a DHFR titration: Decreasing amount of recombinant DHFR were loaded onto lanes of a mini gel separated on SDS-PAGE and blotted with Rabbit Anti-DHFR (C-Terminal) (Cat. No. D0942). Protein loaded per lane ranged from 12.5−200 ng.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.0-1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 0.5-1.0 μg using 100-200 ng of purified DHFR |
| western blot (chemiluminescent): 0.5-1.0 μg/mL using 50-100 ng of purified recombinant DHFR, or 293T human embryonic kidney cell extract, or Jurkat human acute T Cell leukemia cell extract | |
| UniProt accession no. | P00374  |
| Biochem/physiol Actions: | DHFR and DHFR fusion proteins. The epitope resides within amino acids 171-185 of mouse DHFR. |
| Immunogen: | amino acids 171-185 of mouse DHFR, conjugated to KLH via an N-terminal added cysteine residue. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |