Anti-DAP Kinase 1 antibody produced in rabbit
SIGMA/D1319 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DAPK; Anti-DAPK1; Anti-Death associated protein kinase 1
Product Type: Chemical
Immunoblotting HeLa whole cell extract was separated on SDS-PAGE and blotted with Rabbit Anti-DAP-kinase 1 (Cat. No. D1319). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody 1 μg/mL 2. Antibody 1 μg/mL + DAP-kinase 1 immunizing peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~160 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse (predicted), human, rat (predicted) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2.5-5.0 μg using extracts of human HeLa cells |
| western blot: 1-2 μg/mL using whole extracts of human HeLa cells | |
| UniProt accession no. | P53355  |
| Application: | Anti-DAP Kinase 1 antibody is suitable for use in immunoprecipitation (2.5-5.0 μg using extracts of human HeLa cells) and western blot (1-2 μg/mL using whole extracts of human HeLa cells). |
| Application: | Anti-DAP Kinase 1 antibody produced in rabbit has been used in immunoprecipitation and western blotting. |
| Biochem/physiol Actions: | Anti-DAPK1 antibody is specific for human DAPK1 (approx. 160 kDa). In immunoblotting, detection of the DAPK1 band is specifically inhibited with the immunizing peptide. |
| Biochem/physiol Actions: | Death-associated protein kinase (DAPK) belongs to the serine/threonine kinase family that regulates apoptosis, growth factor signaling and also may be involved in modulating immune responses. DAPK1 mediates cellular functions such as membrane blebbing and autophagy and inhibits the activity of prolyl isomerase |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DAP-kinase 1 is a Ca2+/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains, one being Ca2+/calmodulin dependent. |
| Immunogen: | synthetic peptide corresponding to amino acids 2-13 of human DAP-kinase 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |