1-Fluoro-2,4-dinitrobenzene

SIGMA/D1529 - ≥99%

Synonym: 2,4-Dinitro-1-fluorobenzene; DNFB; DNPF; FDNB; Sanger reagent

CAS Number: 70-34-8
Empirical Formula (Hill Notation): C₆H₃FN₂O₄
Molecular Weight: 186.10
EC Number: 200-734-3
MDL Number: MFCD00007056
Linear Formula: (O₂N)₂C₆H₃F
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-D1529-10ML	10 mL	$181.00 1/EA	Add To Favorites
45-D1529-25ML	25 mL	$335.00 1/EA	Add To Favorites
45-D1529-100ML	100 mL	$999.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥99%
bp	178 °C/25 mmHg (lit.)
density	1.482 g/mL at 25 °C (lit.)
form	solid
InChI	1S/C6H3FN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H
InChI key	LOTKRQAVGJMPNV-UHFFFAOYSA-N
mp	25-27 °C (lit.)
Quality Level	200
refractive index	n20/D 1.569 (lit.)
SMILES string	[O-][N+](=O)c1ccc(F)c(c1)[N+]([O-])=O
storage temp.	room temp

Application:	1-Fluoro-2,4-dinitrobenzene has been used to induce severe contact hypersensitivity in mice and to monitor gene expression by in vivo imaging method, and to induce chronic itch as a model for analysing itch-scratching behavior.
Biochem/physiol Actions:	1-Fluoro-2,4-dinitrobenzene is a contact-sensitizing hapten, commonly used in experimental studies on contact hypersensitivity, inducing itch model, and atopic dermatitis. Mast cell activation is a key step in the DNFB induced sensitivity. DNFB covalently binds to the N-terminal amino acid of the protein and aids in Sanger sequencing.
General description:	1-Fluoro-2,4-dinitrobenzene (DNFB) is a reagent that modifies the N-terminal amino acids of proteins and peptides. DNFB is also commonly known as Sanger′s Reagent, named after Frederick Sanger, who reported in a classic 1945 paper the use of DNFB for derivatization of proteins and peptides specifically at their N-termini.†
Packaging:	10, 25, 100 mL in glass bottle

Symbol	GHS07,GHS08
Signal word	Warning
Hazard statements	H302 - H315 - H317 - H319 - H335 - H373
Precautionary statements	P280 - P301 + P312 + P330 - P302 + P352 - P305 + P351 + P338 - P314
Hazard Codes	Xn
Risk Statements	22-33-36/37/38-43
Safety Statements	26-36/37
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	327.2 °F
Flash Point(C)	164 °C

Purity	≥99%
bp	178 °C/25 mmHg (lit.)
mp	25-27 °C (lit.)
Density	1.482 g/mL at 25 °C (lit.)
Refractive Index	n20/D 1.569 (lit.)
Storage Temp.	room temp
UNSPSC	12352204

To Order From a Quote