Anti-Dab-1 (C-terminal) from rabbit
SIGMA/D1569 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Dab1; Anti-Disabled homolog 1; Anti-Yotari
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Dab-1 (C-terminal) from Rabbit (Cat. No. D1569) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Dab-1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Immunoblotting Tissue and cell lysates were separated on SDS-PAGE and blotted with Anti-Dab1 (C-terminal) (Cat. No. D1569). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:1,000. Lanes 1,5. mouse brain 2. rat brain 3. HEK 293T cells transfected with human Dab1 4. HEK 293T cells untransfected 5. Inhibition with Dab1 immunizing peptide (human, 178-194) (2 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~80 kDa |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 2-4 μg/mL using HEK-293T cells expressing human DAB1 |
| western blot: 2-4 μg/mL using mouse and rat brain extracts (S1 fraction) | |
| UniProt accession no. | O75553  |
| Application: | Anti-Dab-1 (C-terminal) was used at a working dilution of 1:2000 to probe the protein sample extracted from brain tissue of mice by western blotting in a study. |
| Biochem/physiol Actions: | Dab1 plays a key role during brain development by controlling neuronal positioning as well as modulation of long-term potentiation (LTP) in the adult brain. Tyrosine phosphorylation of Dab1 is triggered by the binding of reelin to the lipoprotein receptors Apolipoprotein E receptor 2 (ApoER2) and the very low density lipoprotein receptor (VLDLR), thus initiating a signaling cascade that includes the Src-family kinases and Akt. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Disabled-1 is an intracellular adaptor protein that belongs to the Reelin signaling pathway. The amino terminus of Dab1 contains a phosphotyrosine-binding (PTB) domain. The domain interacts with NPXY sequences within the cytoplasmic regions of several membrane-bound proteins including lipoprotein receptors and amyloid precursor protein (APP) family. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |