Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse
SIGMA/D1667 - clone 440.4, ascites fluid
MDL Number: MFCD00164585
Product Type: Chemical
Localization of cytoplasmic dynein to the mitotic spindle in dividing CEF cells. (C) Prometaphase. (F) Metaphase. (I) Anaphase. Immunofluorescent staining of cells using a mixture of Monoclonal Anti-Dynein (Heavy Chain), Clone No. 440.4 and Clone No. 440.1 (Cat. No. D1667) after extraction with a detergent containing microtubule-stabilizing buffer.
From Dr. M.P. Sheetz (Duke University, Durham N.C., USA). Reproduced from Nature, 345, 267 (1990).
From Dr. M.P. Sheetz (Duke University, Durham N.C., USA). Reproduced from Nature, 345, 267 (1990).
Western Blotting Whole extract of Chicken brain were separated on SDS-PAGE and probed with Monoclonal Anti-Dynein (Heavy Chain) antibody produced in Mouse, Clone: 440.4 (Cat. No. D1667). The antibody was developed using 2 μg/mL Anti-Mouse IgG (Fab specific)-Biotin antibody produced in Goat (Cat. No. B0529) and by 2 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Lanes 1. 1:100 antibody 2. 1:200 antibody 3. Negative Control
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 440.4, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG2a |
| mol wt | antigen 440 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, chicken |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: suitable |
| western blot: 1:100 using a chick brain extract or Kinesin enriched rat brain extract | |
| UniProt accession no. | Q8TD57  |
| Application: | Monoclonal Anti-Dynein (Heavy Chain) antibody produced in mouse has been used in: • indirect immunofluorescence at 1:50 dilution • immunocytochemistry • enzyme-linked immunosorbent assay (ELISA) • immunoblotting • dot blot |
| Biochem/physiol Actions: | Dynein has also been implicated in cytoplasmic motile functions, including chromosomal movement, retrograde organelle and axonal transport. It also regulates the endocytic pathway and the organization of the Golgi apparatus. Mutations in dynein axonemal heavy chain 1 (DNAH1) are associated with primary ciliary dyskinesia and multiple morphological anomalies of the flagella (MMAF) that leads to asthenozoospermia and male infertility. |
| Biochem/physiol Actions: | Dynein is a motor protein that regulates the movement of organelles to the ‘minus’ end of microtubules. These motor proteins are also involved in spindle organization, retrograde transport and chromosome movement |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Dynein (Heavy Chain) (mouse IgG2a isotype) is derived from the 440.4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Dynein axonemal heavy chain 1 (DNAH1) encodes for an inner dynein heavy chain. This gene is mapped to the human chromosome location 3p21.1. DNAH1 is expressed in the testis and other ciliated cells. Dyneins are composed of two or three distinct heavy chains, three intermediate chains and at least four light chains. |
| Immunogen: | cytoplasmic dynein from chicken embryo brain. |
| Physical form: | Suppied as ascites fluid with 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |