Anti-DYRK1A (N-terminal) antibody produced in rabbit
SIGMA/D1694 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-
Product Type: Chemical
Immunoblotting Cell and tissue lysates were separated on SDS-PAGE and blotted with Anti-DYRK1A (N-terminal) (Cat. No. D1694). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:1,000. (Lower band at ~84 kDa, may represent endogenous DYRK1A isoform 2) Lanes 1. PC-12 cells 2. Rat embryonic brain (E16) 3. rat embryonic brain (E16) – Inhibition of antibody binding with DYRK1A immunizing peptide (human, 32-51) (10 μg/mL)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~86 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1.5-3 μg/mL using rat brain embryonic extract (S1 fraction) |
| western blot: 3-5 μg/mL using PC12 cell lyste | |
| UniProt accession no. | Q13627  |
| Application: | Anti-DYRK1A (N-terminal) antibody produced in rabbit has been used in immunoblotting and immunohistochemistry. |
| Biochem/physiol Actions: | DYRK1A is encoded by a gene located within the Down syndrome (DS) critical region on human chromosome 21 and its expression is found to be elevated in individuals with DS. It might be one of the genes involved in some of the neurological abnormalities observed in DS. It phosphorylates several substrates including transcription factor FKHR, NFAT, STAT3, microtubule-associated protein Tau, glycogen synthase and c-AMP-response element-binding protein. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DYRK1A (dual-specificity tyrosine-phosphorylated regulated kinase 1A) is a member of a growing family of Ser/Thr protein kinases termed DYRKs. |
| General description: | DYRK1A (N-terminal) encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-c |
| General description: | The human and rodent DYRK1A are ubiquitously expressed in adult and fetal tissue with high expression in the brain and heart during development. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |