DAF-FM
SIGMA/D1821 - ≥98% (HPLC)
Synonym: 4-
CAS Number: 254109-20-1
Empirical Formula (Hill Notation): C21H14F2N2O5
Molecular Weight: 412.34
MDL Number: MFCD29073049
Linear Formula: C21H14F2N2O5
Product Type: Chemical
| assay | ≥98% (HPLC) |
| color | white to brown |
| form | powder |
| InChI | 1S/C21H14F2N2O5/c1-25-13- |
| InChI key | DIJCILWNOLHJCG-UHFFFAOYSA |
| Quality Level | 100  |
| shipped in | wet ice |
| SMILES string | CNc1ccc2c(c1N)C(=O)OC23c4 |
| solubility | DMSO: >1 mg/mL |
| storage condition | desiccated |
| storage temp. | −20°C |
| Application: | DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells. |
| Biochem/physiol Actions: | DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). |
| Biochem/physiol Actions: | DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes. |
| Disclaimer: | hygroscopic |
| Packaging: | 1 mg in glass bottle |
| Preparation Note: | Color variation within the range from white to brown does not change performance. |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |