Synonym: 4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one; Diaminofluorescein-FM diacetate
CAS Number: 254109-22-3
Empirical Formula (Hill Notation): C25H18F2N2O7
Molecular Weight: 496.42
MDL Number: MFCD16872050
Linear Formula: C25H18F2N2O7
Product Type: Chemical
| assay |
≥98% (HPLC) |
| color |
colorless to yellow |
| fluorescence |
λex 500 nm; λem 515 nm in DMSO |
| form |
film |
| InChI |
1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3 |
| InChI key |
BEVHTVRRVVEMEF-UHFFFAOYSA-N |
| Quality Level |
100  |
| shipped in |
wet ice |
| SMILES string |
CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35 |
| storage condition |
desiccated |
| storage temp. |
−20°C |
| Application: |
DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs). |
| Biochem/physiol Actions: |
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). |
| Biochem/physiol Actions: |
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases. |
| Disclaimer: |
Hygroscopic |
| Features and Benefits: |
This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here . |
| Packaging: |
1 mg in glass bottle |
| RIDADR |
NONH for all modes of transport |
| WGK Germany |
WGK 3 |
| Flash Point(F) |
Not applicable |
| Flash Point(C) |
Not applicable |
| Purity |
≥98% (HPLC) |
| Storage Temp. |
−20°C |
| UNSPSC |
12352200 |
