Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse
SIGMA/D2178 - clone DAPK-55, ascites fluid
Synonym: Anti-DAP Kinase
MDL Number: MFCD00282220
Product Type: Chemical
Western Blotting JOURNAL CITATION: Tumour suppressor death-associated protein kinase targets cytoplasmic HIF-1α for Th17 suppression. By: Chou, T. F., Chuang, Y. T., et al. in Nat Commun, 2016. PubMed ID: 27312851 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/ncomms11904), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunoblotting Whole extract of rat brain was separated on SDS-PAGE and probed with 1:500 Monoclonal Anti-Death Associated Protein Kinase Clone: DAPK-55 (Cat. No. D2178). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DAPK-55, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| mol wt | antigen 160 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): suitable |
| microarray: suitable | |
| western blot: 1:250 using COS-7 cell extract | |
| UniProt accession no. | P51397  |
| Application: | Monoclonal Anti-Death Associated Protein Kinase antibody produced in mouse has been used in immunoblotting and immunoprecipitation. |
| Biochem/physiol Actions: | DAP-kinase is a novel, structurally unique, human serine/threonine kinase identified as a positive mediator of interferon-γ-induced programmed cell death. It has an intrinsic kinase activity towards itself that is regulated by calmodulin and calcium. |
| Biochem/physiol Actions: | Death-associated protein 1 (DAP1) participates in the regulation of cell growth and death including that of cancer cells. DAP-kinase has an intrinsic kinase activity towards itself that is regulated by calmodulin and calcium. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-Death Associated Protein (DAP) Kinase (mouse IgG1 isotype) is derived from the DAPK-55 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Death-associated protein 1 (DAP1) gene is mapped to human chromosome 9q34.1. It belongs to the DAP family. |
| General description: | The gene is widely expressed in many tissues of embryonic and adult origin and gives rise to a single 6.3 Kb mRNA transcript. The protein consists of 1423 amino acids (160 kDa) and belongs to a family of calmodulin-dependent protein kinases. It contains 8 ankyrin repeats and 2 potential P-loop motifs apart from the kinase domain and the calmodulin regulatory domain. |
| Immunogen: | recombinant human DAP kinase expressed in bacteria. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |