Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse
SIGMA/D218 - clone 1A, buffered aqueous solution
MDL Number: MFCD01633297
Product Type: Chemical
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| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| clone | 1A, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~200 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human (weakly), guinea pig, mouse, rat, rabbit |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): 1:200 |
| immunoprecipitation (IP): suitable | |
| western blot (chemiluminescent): 1:500 | |
| UniProt accession no. | Q13698  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Application: | Monoclonal Anti-Dihydropyridine Receptor (α1 Subunit) antibody produced in mouse is suitable for immunoprecipitation, immunohistochemistry at a working dilution of 1:500 and immunoblotting at 1:2000. It was used for the detection and localization of DHPR-1α by western blotting in skeletal muscle tissue sections of mice in a study. The antibody may be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells. |
| Biochem/physiol Actions: | Reacts specifically with the α1 subunit of the DHP receptor. May be used to inhibit the DHP-sensitive calcium current in BC3H1 mouse muscle cells. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines. |
| Biochem/physiol Actions: | The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type Ca2+ channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | DHP receptor is composed of at least four subunits. The α1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated Na+ channel. The α2 subunit is a large glycoprotein and the other 2 subunits are termed β and γ. |
| Immunogen: | rabbit muscle transverse tubule dihydropyridine (DHP) receptors. |
| Physical form: | Solution in phosphate buffered saline containing 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |