Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse
SIGMA/D219 - clone 20A, ascites fluid, buffered aqueous solution
Synonym: Anti-CACNA2; Anti-CACNL2A; Anti-CCHL2A; Anti-LINC01112; Anti-lncRNA-N3
MDL Number: MFCD01323189
Product Type: Chemical
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| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | 20A, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen 143 kDa (reduced) |
| antigen 220 kDa (non-reduced) | |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, rabbit, guinea pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (frozen sections): 1:500 |
| western blot: 1:500 | |
| UniProt accession no. | O08532  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Application: | Monoclonal Anti-Dihydropyridine Receptor (α2 Subunit) antibody produced in mouse has been used in: • immunofluorescent histochemistry • surface plasmon resonance binding • immunohistochemistry (IHC) • immunoblotting |
| Application: | Use of this antibody for immunoblot analysis will allow for detection of a 220 kDa band (native conditions) or a 143 kDa band (denaturing conditions).Use of this antibody for immunohistochemistry of muscle tissue will demonstrate double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines are observed. |
| Biochem/physiol Actions: | Dihydropyridine Receptor (DHFR) α1 subunit is the voltage-sensor. DHPR interacts with ryanodine receptor (RyR) and also mediates excitation-contraction (EC) coupling in cardiac and skeletal muscles. Mutation in the DHPR (E1014K) affects its affinity and selectivity towards calcium permeation. Additionally, it also plays a key role in the synaptic transmission. |
| Biochem/physiol Actions: | Reacts specifically with the α2 subunit of the DHP receptor. Strong reactivity is observed with the α2 subunit in rat, mouse, guinea pig, rabbit skeletal and cardiac muscle, and rat brain and lung tissue. Weaker reactivity is observed with rat kidney, liver and spleen tissues. By immunohistochemistry, double rows of discrete punctate staining represent pairs of triads on the opposing sides of the Z-lines. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Dihydropyridine Receptor (DHPR) is a heteropentamer calcium ion channel and comprises of α1,α2/δ,β and γ subunits. It is localized in the skeletal muscle in the t-tubule membrane. The α2subunit of DHPR is a glycosylated subunit and is anchored on the membrane. |
| Immunogen: | Rabbit dihydropyridine (DHP) receptors, (α2 subunit) |
| Other Notes: | This mouse monoclonal IgG2a antibody is supplied as a mouse ascites diluted in phosphate buffered saline containing 0.05% sodium azide as a preservative. |
| Physical form: | Solution in phosphate buffered saline containing 0.05% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |