Anti-Derlin-3 antibody produced in rabbit
SIGMA/D2194 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DERL3; Anti-Der1-like domain family, member 3
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 20 μg/mL Rabbit Anti-Derlin-3 (Cat. No. D2194). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoprecipitation Rabbit Anti-Derlin-3 (Cat. No. D2194) was used to immunoprecipitate Derlin-3 from HEK-293T overexpressing human Derlin-3 whole cell extract. Detection antibody: Mouse Anti-c-Myc-antibody (Cat. No. M4439) Lanes 1. 2 μg antibody 2. 5 μg antibody 3. 5 μg non-relevant antibody
Immunoblotting Whole extract of HEK-293T cells overexpressing human Derlin-3 was separated on SDS-PAGE and probed with Rabbit Anti-Derlin-3 (Cat. No. D2194). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 5 μg/mL antibody 2. 5 μg/mL antibody + Derlin-3 immunizing peptide (human, 192-205)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~26 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat (predicted), human, mouse (predicted) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using extract of HEK-293T cells expressing recombinant human Derlin-3 |
| indirect immunofluorescence: 10-20 μg/mL using human HeLa cells | |
| western blot: 2-5 μg/mL using whole extract of HEK-293T cells expressing recombinant human Derlin-3 | |
| UniProt accession no. | Q96Q80  |
| Application: | Anti-Derlin-3 antibody produced in rabbit has been used in immunoblotting, immunoprecipitation, and immunofluorescence. |
| Biochem/physiol Actions: | Derlin-3 is involved in the degradation of misfolded glycoproteins in the ER. Derlin-3 shares ~30% sequence identity with Derlin-1 and spans the lipid bilayer of the ER four times, showing structural similarity to Derlin-1. It is a component of the mammalian ER-associated degradation (ERAD) mechanism and is upregulated by unfolded protein response (UPR). Overexpression of this gene leads to increase in degradation of misfolded glycoprotein, whereas its knockdown blocks degradation. Derlin-3 also interacts with the mammalian orthologs of the yeast Hrd1p/Hrd3p ubiquitin-ligase complex. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Derlin-3 is a mammalian homologues of yeast Der1p, a transmembrane protein required for yeast endoplasmic reticulum (ER)-associated degradation (ERAD). Derlin 3 is encoded by the gene mapped to human chromosome 22q11.23. It is expressed in the ER and is a member of the derlin protein family. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 192-205 of human Derlin-3, conjugated to KLH. The corresponding sequence differs by 3 amino acids in mouse and 2 amino acids in rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |