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DAF-FM solution

SIGMA/D2196 - 5 mM in DMSO, ≥97% (HPLC)

Synonym: 4-Amino-5-methylamino- 2′,7′-difluorofluorescein; Diaminofluorescein-FM

CAS Number: 254109-20-1
Empirical Formula (Hill Notation): C21H14F2N2O5
Molecular Weight: 412.34
MDL Number: MFCD29073049
Linear Formula: C21H14F2N2O5
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-D2196-1MG 1 mg
 $812.00
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assay ≥97% (HPLC)
color colorless to yellow, including pink
form DMSO solution
InChI 1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3
InChI key DIJCILWNOLHJCG-UHFFFAOYSA-N
Quality Level 100 
shipped in wet ice
SMILES string Fc1cc2c(cc1O)Oc3c(cc(c(c3)O)F)C42OC(=O)c5c4ccc(c5N)NC
storage condition desiccated
storage temp. −20°C
Application: DAF-FM solution has been used as a fluorescent probe for detecting nitric oxide (NO) from plant samples.
Biochem/physiol Actions: DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).
Biochem/physiol Actions: DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM (Ex: 500 nm; Em: 515 nm; dectection limit: 3 nM) is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, but it does not need to be activated by cytosolic enzymes.
Features and Benefits: This compound is featured on the Nitric Oxide Synthases  page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here .
Packaging: 1 mg in glass bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥97% (HPLC)
Storage Temp. −20°C
UNSPSC 12352200

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