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Diaphorase from Clostridium kluyveri

SIGMA/D2197 - recombinant, expressed in E. coli, Animal-component free

Synonym: Diaphorase; Lipoamide Dehydrogenase; Lipoyl Dehydrogenase

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-D2197-100UN 100 units
 $85.20
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45-D2197-300UN 300 units
 $223.00
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45-D2197-500UN 500 units
 $286.00
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Unit Definition One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor. Stoichiometry: protein N6-(dihydrolipoyl)lysine + NAD+ → protein N6-(lipoyl)lysine + NADH + H+
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D2197-300UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D2197-500UN

 

form solid
Quality Level 200 
recombinant expressed in E. coli
specific activity >=30 units/mg protein (biuret)
storage temp. −20°C
Application: Diaphorase from Clostridium kluyveri has been used:• in lactate dehydrogenase assay in gill cells.
  • impregnated on the strip for formate dehydrogenase assay.
  • in various assays to oxidate β-NADH or β-NADPH in the presence of an electron acceptor.
  • for semiquinone generation during benzoquinone reduction.
    • for evaluation of glucose uptake in cultured muscle cells.
  • in lactate dehydrogenase cytotoxicity assay.
    		•
    Biochem/physiol Actions: Diaphorase catalyzes the oxidation of either β-NADH or β-NADPH in the presence of an electron acceptor such as methylene blue or 2,6-dichlorophenolindophenol. Diaphorases that are specific for either β-NADH or β-NADPH are known. The pig heart enzyme of Straub seems to have native diaphorase (β-NADH specific) as well as lipoic and lipoamide dehydrogenase activities.
    General description: Research area: Cancer
    Other Notes: One unit of diaphorase will oxidize 1.0 micromole of beta-NADH per minute at pH 7.5 at 25 degrees C, with the corresponding reduction of the appropriate electron acceptor.
    Packaging: 100, 300, 500 units in poly bottle
    Physical form: Supplied as a lyophilized powder containing trehalose.
    RIDADR NONH for all modes of transport
    WGK Germany WGK 3
    Flash Point(F) Not applicable
    Flash Point(C) Not applicable
    activity specific activity: >=30 units/mg protein (biuret)
    Storage Temp. −20°C
    Enzyme Commission (EC) Number 1.8.1.4   ( BRENDA  | IUBMB  )
    UNSPSC 12352204

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