Denhardt’s Solution 50x
SIGMA/D2532 - for Northern and Southern blotting, solution
Synonym: blocking reagent; blocking solution; denhardts; hybridization buffer; hybridization solution; northern blocking solution; pre-hybridization; southern blocking solution
Product Type: Chemical
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| foreign activity | DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected |
| form | solution |
| Quality Level | 300  |
| storage temp. | −20°C |
| technique(s) | Southern blotting: suitable |
| General description: | Denhardt’s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers. |
| Preparation Note: | Denhardt’s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H317 - H350 |
| Precautionary statements | P202 - P261 - P264 - P280 - P302 + P352 - P308 + P313 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12161700 |