Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit
SIGMA/D2693 - affinity isolated antibody, buffered aqueous glycerol solution, sufficient for 10 blots
MDL Number: MFCD08705496
Product Type: Chemical
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| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| mol wt | antigen 32 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr75) |
| technique(s) | dot blot: 1:1,000 |
| western blot: 1:1,000 | |
| UniProt accession no. | Q9UD71  |
| usage | sufficient for 10 blots |
| Application: | Anti-phospho-DARPP32 (pThr75) antibody produced in rabbit is suitable for dot blot and western blot analysis at a working dilution of 1:1000 using rat caudate lysates. |
| Biochem/physiol Actions: | Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32. Phosphorylation of DARPP32 plays an important role in the regulation of dopaminergic neurotransmission. The activity of this protein has an important role in the actions of alcohol, caffeine and Prozac®. |
| General description: | DARPP32 is a phosphoprotein with a molecular mass of 32kDa. It is regulated by dopamine (DA) and cAMP and is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. DARPP32 is a target for the actions of dopamine. |
| General description: | DARPP32 phosphorylated on Thr75 acts as an inhibitor of PKA. |
| Immunogen: | synthetic phosphopeptide corresponding to amino acids residues surrounding the DARPP32 phosphorylated on threonine 75. |
| Legal Information: | Prozac is a registered trademark of Eli Lilly and Co. |
| Other Notes: | The labeling by the antibody to DARPP32 Thr75 is markedly increased by okadaic acid treatment. |
| Physical form: | Solution at 100 μL in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |