Deoxyribonuclease I bovine
SIGMA/D2821 - recombinant, expressed in Pichia pastoris, lyophilized powder, RNAse and protease, free
Synonym: DNAse I; Deoxyribonucleate 5′-oligonucleotido-hydrolase
MDL Number: MFCD00130918
Product Type: Chemical
SDS-PAGE Deoxyribonuclease I Bovine (Cat. No. D2821) was assessed on SDS-PAGE.
| application(s) | diagnostic assay manufacturing |
| biological source | bovine |
| foreign activity | RNAse and protease, free |
| form | lyophilized powder |
| mol wt | ~39 kDa |
| Quality Level | 300  |
| recombinant | expressed in Pichia pastoris |
| solubility | H2O: soluble (pH 4.0-9.0) |
| specific activity | ≥4,000 units/mg protein |
| storage temp. | 2-8°C |
| suitability | suitable for molecular biology |
| technique(s) | DNA extraction: suitable |
| Application: | Deoxyribonuclease I bovine has been used in a study to investigate the inhibition of DNA polymerase by extracts of rat liver. Deoxyribonuclease I bovine has also been used in a study to investigate the effects of ionic strength on enzymic activity. |
| Application: | Deoxyribonuclease I bovine has been used in the preparation of cold cell lysis buffer, complete RNA lysis buffer, cell lysis buffer for testing the expression of recombinant tagged protein. |
| Application: | The enzyme from Sigma has been used for the digestion of DNA extracted from Agave spp. clones. The enzyme was used during a study that investigated the effect of epigenetic changes on the regulatory expression of KNOTTED1-like HOMEOBOX (KNOX) transcription factors. |
| Application: | Used for the removal of DNA from protein samples. |
| Biochem/physiol Actions: | Digests single- and double-stranded DNA to a mixture of mono- and oligonucleotides carrying 5′ phosphates and 3′ OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved. |
| Biochem/physiol Actions: | DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity. |
| Features and Benefits: | • RNA purification by removing DNA • Prepare DNA for nick translation1 • Footprinting assays to determine DNA-protein interactions2 |
| Other Notes: | One unit will produce a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA at pH 5.0 and 25°C |
| Physical form: | supplied as a lyophilized powder containing glycine as a stabilizer |
| Preparation Note: | Produced without using any animal cells or animal derived materials. |
| Preparation Note: | The enzyme powder may be reconstituted in water or any buffer at pH 4.0-9.0, except phosphate buffer. Calcium chelators should be avoided. 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at –20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for upto five hours at 60 °C and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4,000 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.1.21.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |