DNA Ligase from T4-infected Escherichia coli
SIGMA/D2886 - buffered aqueous glycerol solution
Synonym: Polydeoxyribonucleotide Synthase; Polynucleotide Ligase; T4 DNA Ligase
CAS Number: 9015-85-4
EC Number: 232-770-0
MDL Number: MFCD00163508
Product Type: Chemical
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| form | buffered aqueous glycerol solution |
| grade | Molecular Biology |
| mol wt | 68 kDa |
| Quality Level | 200  |
| specific activity | 4,000 U/mL |
| storage temp. | −20°C |
| UniProt accession no. | P00970  |
| Application: | Suitable for: • Ligation of blunt ended or cohesive DNA fragments • Ligation of cloning vector and restriction insert fragments • Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids • Couple RNA single strands by bridging oligonucleotide adapters |
| Biochem/physiol Actions: | T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3′ hydroxyl and 5′ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks. |
| Other Notes: | One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of P32 from pyrophosphate into ATP as Norit-absorbable material in 20 minutes at 37°C. |
| Other Notes: | T4 DNA Ligase is inactivated by heating at 65 °C for 10 minutes. |
| Other Notes: | T4 DNA Ligase is supplied in a solution containing 20 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, and 50% (v/v) glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 4,000 U/mL |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 6.5.1.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |