Lambda DNA Mixed Digest
SIGMA/D2916 - for DNA electrophoresis
Product Type: Chemical
Representative product tube
| form | buffered aqueous solution |
| Quality Level | 100  |
| 200 |
|
| storage temp. | −20°C |
| suitability | suitable for electrophoresis (DNA) |
| usage | 100 uses (0.1 mg size) |
| 20 uses (20 μg size) |
| Application: | Lambda DNA Mixed Digest has been used for DNA electrophoresis. |
| Application: | Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels. |
| General description: | Sigma’s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. |
| Other Notes: | For optimal resolution, the recommended agarose gel concentration is 0.4%. |
| Other Notes: | Sigma’s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA. |
| Preparation Note: | Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |