Anti-D1 Dopamine Receptor antibody,Rat monoclonal
SIGMA/D2944 - clone 1-1-F11 s.E6, purified from hybridoma cell culture
Synonym: Anti-DRD1
MDL Number: MFCD00282304
Product Type: Chemical
Immunofluorescence
Human HEK-293T cells transfected with D1 dopamine receptor were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. The cells were incubated with Monoclonal Anti-D1 dopamine receptor (clone: 1-1-F-11 SE.6) (Cat. No. D2944) and further developed with FITC-conjugated Rabbit Anti-Rat IgG (A) and DAPI (B).
Human HEK-293T cells transfected with D1 dopamine receptor were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. The cells were incubated with Monoclonal Anti-D1 dopamine receptor (clone: 1-1-F-11 SE.6) (Cat. No. D2944) and further developed with FITC-conjugated Rabbit Anti-Rat IgG (A) and DAPI (B).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rat |
| clone | 1-1-F11 s.E6, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG2a |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, monkey, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 5-10 μg/mL using D1 dopamine receptor transfected HEK-293T cells |
| immunohistochemistry: suitable | |
| western blot: suitable | |
| UniProt accession no. | P21728  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) Immunohistochemistry (1 paper) |
| Application: | Monoclonal Anti-D1 Dopamine Receptor antibody produced in rat has been used in: • immunohistochemistry • immunoblotting • immunocytochemistry • proximity ligation assay • co-immunoprecipitation • western blotting |
| Biochem/physiol Actions: | D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. D1 receptors are expressed on airway smooth muscle (ASM) and regulate smooth muscle force via cAMP activation of protein kinase A. It is a potential target for therapeutic relaxation of ASM. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | D1 Dopamine Receptor encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. |
| General description: | Monoclonal Anti-D1 Dopamine Receptor (rat IgG2a isotype) is derived from the rat hybridoma 1-1-F11 S.E6 produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor. Dopamine receptors belong to the family of seven transmembrane domain G protein coupled receptors (GPCRs). Dopamine receptors are mainly localized in the striatum, limbic system, the brain cortex and the infundibulum. The D1-like family consists of the D1 and D5 receptors. |
| Immunogen: | recombinant fusion protein containing the C-terminal 97 amino acid of human D1 dopamine receptor. |
| Legal Information: | Sold under exclusive license from Emory University |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |