Deoxyribonucleic acid from human placenta
SIGMA/D3035 - buffered aqueous solution, sexed, female
Synonym: DNA
CAS Number: 9007-49-2
MDL Number: MFCD00130921
Product Type: Chemical
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| biological source | human placenta |
| form | buffered aqueous solution |
| grade | Molecular Biology |
| InChI | 1S/C15H31N3O13P2/c16-13-1 |
| InChI key | AWBASQCACWFTGD-UHFFFAOYSA |
| Quality Level | 200  |
| shipped in | dry ice |
| SMILES string | [P](=O)(OC2C(OC(C2)N)CO[P |
| storage temp. | −20°C |
| Application: | Deoxyribonucleic acid from human placenta has been used: • to compare the efficiency of DNA quantification methods • as a standard in real-time PCR analysis of DNA samples from bladder cancer cell lines • as an internal control to estimate the degree of fragmentation of circulating DNA • for restriction digest analysis to identify low copy repeat regions of human chromosome 22q11 • to compare the 70-bp P5 exon sequence between DNA of different human and primate species • to calculate copy number of genes, relative to normal human tissue • in PCR reactions |
| Application: | For use in Southern hybridizations. |
| General description: | Human placental DNA is isolated from a single female donor placenta, but will contain some maternal DNA. The sex has been determined using hybridization with DNA probes. |
| Other Notes: | Solutions of DNA have been stored successfully for several months at 4 C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent. At low concentrations, about 25 μg/ml, DNA tends to absorb onto the surfaces of plastic tubes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106310 |