Anti-DVL1 (C-terminal) antibody produced in rabbit
SIGMA/D3320 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Dishevelled-1; Anti-Dsh homolog 1; Anti-Segment polarity protein dishevelled homolog
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-DVL1 (C-terminal) antibody produced in Rabbit (Cat. No. D3320) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing DVL1 (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1.5 μg/mL.
Immunoblotting Cell lysates of HEK-293T overexpressing human DVL1 (lanes 1,3) and of HEK-293T non transfected (lane 2), were separated on SDS-PAGE, blotted with Anti-DVL1 (C-terminal) (Cat. No. D3320) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and chemiluminescent substrate. Lanes 1. Antibody: 1.5 μg/mL 2. Antibody: 1.5 μg/mL 3. Antibody: 1.5 μg/mL + DVL1 immunizing peptide (human, 592-609)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~85 kDa |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 2.0-4.0 μg/mL using HEK-293T cell lysate expressing human DVL1 |
| UniProt accession no. | O14640  |
| Application: | Anti-DVL1 (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence. |
| Biochem/physiol Actions: | Dishevelled (Dsh, DVL) operates by up-regulating β-catenin levels and stimulating T-cell factor (TCF)/lymphoid enhancer-binding factor 1 (LEF-1)-dependent transcription. In mammals, three genes encoding isoforms of dishevelled are present, DVL1, DVL2, and DVL3, that differentially mediate the wingless (wnt) canonical signaling pathway. |
| Biochem/physiol Actions: | Dishevelled (Dsh, DVL) proteins are part of a multigene family that mediate wnt signaling pathways that are essential for the regulation of cellular proliferation, differentiation, motility, and morphogenesis. DVL1 regulates the activity of JNK (c-Jun N-terminal kinase) and GSK3β in the wnt signaling pathway. Upon activation of the wnt signaling pathway, DVL1 inactivates GSK3β through complex formation with APC (Antigen-presenting cell), β-catenin and axin proteins, releasing β-catenin from degradation. In neurons, DVL1 is localized to axonal microtubules and stabilizes microtubules by inhibiting the GSK3β mediated phosphorylation of MAP-1B. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The three DVL isoforms display high sequence homology and have conserved DIX, PDZ and DEP domains required for GSK3β inactivation. DVL1 is highly expressed in adult and fetal tissues such as skeletal muscle and pancreas but is also found in the brain and neural tube. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |