Dipeptidyl Peptidase IV human
SIGMA/D3446 - recombinant, expressed in Sf9 cells
Synonym: CD26; DPPIV; Dipeptidyl aminopeptidase IV; Glycoprotein GP110
MDL Number: MFCD00166456
Product Type: Chemical
Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. Both the human and porcine kidney enzymes exist as homodimers with a subunit molecular mass of ~30 kDa. Human recombinant DPPIV (Cat. No. D4943) is supplied as the 105 kDa soluble form representing amino acid residues 29-766 with a C-terminal histidine tag.
| concentration | ≥0.01 mg/mL |
| form | solution |
| mol wt | 124 kDa |
| Quality Level | 200  |
| recombinant | expressed in Sf9 cells |
| relevant disease(s) | cancer (lymphoma, prostate and colon cancer) |
| shipped in | dry ice |
| specific activity | ≥4,000 units/μg protein |
| storage temp. | −70°C |
| UniProt accession no. | P27487  |
| Application: | Dipeptidyl Peptidase IV human has been used in DPP-IV inhibitory activity assay of quinoa protein concentrate digest. |
| Application: | Human dipeptidyl peptidase IV has been used in a study to assess the interactive hemodynamic effects of its inhibition as well as the angiotensin-converting enzyme inhibition. Human dipeptidyl peptidase IV has also been used in a study to identify and characterize the 100 kDa High Molecular Weight Hymenoptera Venom Allergens Api m 5 and Ves v 3. |
| Biochem/physiol Actions: | Dipeptidyl Peptidase IV (DPP4) mediates the activation of T-cells. It is also a potential biomarker for lymphoma, thyroid, prostate and colon cancer. |
| Biochem/physiol Actions: | DPPIV has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes N-terminal dipeptides from the unsubstituted N-terminus of peptides with the sequence of X-Pro-Z and X-Ala-Z. Where X is a nonspecific residue at the N terminus and Z cannot be proline or hydroxyproline. |
| Biochem/physiol Actions: | Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. There is an 88% sequence homology between the human and porcine kidney enzymes. Both exist as homodimers with a subunit molecular weight of ~30 kDa. The high mannose 100 kDa DPPIV precursor is processed in the Golgi to yield a 124 kDa heavily N-and O-linked mature glycoprotein. It is then sorted to the apical membrane through the concerted action of both N- and O-linked glycans and its association with lipid microdomains. The porcine enzyme contains 18.3% carbohydrates, which the glycan composition is 0.9% fucose, 3.4% mannose, 5.1% galactose, 8.2% glucosamine, and 0.7% sialic acid. DPPIV is highly expressed on endothelial cells, epithelial cells, and lymphocytes. It is also present in plasma in its soluble form. |
| General description: | Dipeptidyl Peptidase IV (DPP4) is mapped to human chromosome 2q24.2. It comprises the N-glycosylation sites located in the β-propeller domain. |
| Other Notes: | One unit will hydrolyze 1.0 picomole of Ala-Pro-AMC per minute at pH 7.5 at 25 deg °C |
| Other Notes: | View more information on Dipeptidyl Peptidase IV at www.sigma-aldrich.com/enz. |
| Physical form: | Supplied as a solution in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole and 10% glycerol. |
| Symbol |   GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H315 - H319 - H360D |
| Precautionary statements | P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61 |
| Safety Statements | 53-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥4,000 units/μg protein |
| Storage Temp. | −70°C |
| Enzyme Commission (EC) Number | 3.4.14.5 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |