Dipeptidyl Peptidase III human
SIGMA/D3571 - recombinant, expressed in Sf9 cells
Synonym: DPP III
Product Type: Chemical
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| concentration | ≥0.1 mg/mL |
| form | solution |
| mol wt | 82 kDa |
| NCBI accession no. | NM_005700   |
| NP_005691 |
|
| Quality Level | 200 |
| recombinant | expressed in Sf9 cells |
| shipped in | dry ice |
| specific activity | ≥400 units/μg protein |
| storage temp. | −70°C |
| Application: | Human dipeptidyl peptidase III has been used in a study to assess the effect of entropy-driven binding of opioid peptides on large domain motion in human dipeptidyl peptidase III. Human dipeptidyl peptidase III has also been used in a study to investigate Ets-1/Elk-1 as a critical mediator of its transcription in human glioblastoma cells. |
| Biochem/physiol Actions: | DPP III is a cytosolic zinc-exopeptidase that is involved in the intracellular protein catabolism of eukaryotes. The enzyme is a monomeric acidic protein with a molecular mass of approximately 82,000 Da and a pI of 4.5-4.6. It is sensitive to freezing and temperatures above 40 °C. It is found to be inhibited by metallo-chelators and sulfydryl reagents. The activity can be restored by divalent cations and thiol compounds. It has a particularly high affinity for angiotensin III. It acts as a post-proline-cleaving enzyme on endomorphins. |
| Other Notes: | One unit will hydrolyze 1.0 picomole of Arg-Arg-AMC per minute at pH 7.5 at 25 deg °C |
| Physical form: | Supplied as a solution in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 10% glycerol and 3 mM DTT. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥400 units/μg protein |
| Storage Temp. | −70°C |
| UNSPSC | 12352204 |