DirectLoad™ 50 bp DNA Step Ladder
SIGMA/D3812 - ready-to-use marker for DNA electrophoresis
Synonym: DirectLoad™ 50 bp DNA Marker; DirectLoad™ 50 bp DNA Marker; Ready-to-use 50 bp DNA Marker; Ready-to-use 50 bp DNA Marker; Ready-to-use DirectLoad™ 50 bp DNA Marker; Ready-to-use DirectLoad™ 50 bp DNA Marker; Ready-to-use DirectLoad™ 50 bp DNA Step Ladder; Ready-to-use DirectLoad™ 50 bp DNA Step Ladder
Product Type: Chemical
D3812 on a 1.5% agarose gel
| form | (liquid) |
| Quality Level | 200  |
| storage temp. | −20°C |
| suitability | suitable for electrophoresis (DNA) |
| usage | sufficient for 100 loads |
| Application: | DirectLoad™ 50 bp DNA Step Ladder has been used for cell lysis and genomic cleavage detection assay. |
| Application: | Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels. |
| Features and Benefits: | • Ready-to-load • Easy-to-use • Popular band sizes |
| General description: | Sigma’s DirectLoad™ 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel. |
| Legal Information: | DirectLoad is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | For optimal resolution, the recommended agarose gel concentration is 2.0%. |
| Other Notes: | Sigma’s DirectLoad™ 50 bp DNA Step Ladder is provided in a gel-loading solution of 5% glycerol, 4.2 mM EDTA, 0.083% orange G and 0.0125% xylene cyanol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41105335 |