Anti-Drebrin antibody produced in rabbit
SIGMA/D3816 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-
MDL Number: MFCD03845304
Product Type: Chemical
Immunoblotting
Whole HeLa cell extracts (lane 1) and MCF7 cells (lane 2) were separated on SDS-PAGE and blotted with Rabbit Anti-Debrin (Cat. No.D3816) and developed with Goat Anti-Rabbit IgG-Alkaline Phosphatase (Cat. No. A9919) and SIGMA FAST™ BCIP/NBT substrate (Cat. No. B5655).
Whole HeLa cell extracts (lane 1) and MCF7 cells (lane 2) were separated on SDS-PAGE and blotted with Rabbit Anti-Debrin (Cat. No.D3816) and developed with Goat Anti-Rabbit IgG-Alkaline Phosphatase (Cat. No. A9919) and SIGMA FAST™ BCIP/NBT substrate (Cat. No. B5655).
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:1,000 using rat brain cytosol extract |
| western blot: 1:1,000 using whole extract of the human epitheloid carcinoma HeLa cell line | |
| UniProt accession no. | Q16643  |
| Biochem/physiol Actions: | Drebrin (developmentally-regulate |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Drebrin is a member of the newly identified ADF-H family of actin-binding proteins that share the structurally conserved actin-depolymerizing factor (ADF) binding module. It is composed of a single ADF-H domain at its N-terminus, followed by a non-conserved central region and a C-terminal SH3 domain. Three isoforms, E1 and E2 (embryonic) and A (adult), have been identified in the brain. An additional truncated form of drebrin A, s-drebrin/A2, is specifically expressed in the adult brain, but not in non-neuronal tissues. Drebrin is also widely expressed in a variety of cells including epithelial, endothelial, and smooth muscle cells and is associated at cell-cell adhering junction sites. |
| Immunogen: | synthetic peptide corresponding to the N-terminal region of human drebrin E/A (amino acids 22-42) conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |