Deoxyribonuclease II from porcine spleen
SIGMA/D4138 - Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)
Synonym: DNase II; Deoxyribonucleate 3′-oligonucleotido-hydrolase
CAS Number: 9025-64-3
EC Number: 232-801-8
MDL Number: MFCD00130919
Product Type: Chemical
Catalyzes the endonucleolytic cleavage of double and single stranded DNA to yield nucleoside 3′-phosphates and 3′-phosphooligonucleotide end-products.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D4138-80KU
| composition | Protein, 60-80% |
| foreign activity | RNase ≤0.05% |
| form | lyophilized powder |
| mol wt | 45 kDa |
| Quality Level | 200  |
| specific activity | 2,000-6,000 Kunitz units/mg protein (biuret) |
| storage temp. | −20°C |
| type | Type IV |
| Application: | Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane. |
| Application: | DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA). |
| Biochem/physiol Actions: | The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions. It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass. |
| Other Notes: | One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25°C; [Mg2+] = 0.83 mM |
| Packaging: | 20000, 80000 units in poly bottle |
| Physical form: | Contains sodium chloride |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 2,000-6,000 Kunitz units/mg protein (biuret) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.22.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |