Plasmid DNA from E. coli RRI
SIGMA/D4154 - pUC18, buffered aqueous solution
CAS Number: 9007-49-2
MDL Number: MFCD00166896
Product Type: Chemical
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| biological source | Escherichia coli (RRI) |
| form | buffered aqueous solution |
| grade | Molecular Biology |
| InChI | 1S/C15H31N3O13P2/c16-13-1 |
| InChI key | AWBASQCACWFTGD-UHFFFAOYSA |
| Quality Level | 200  |
| shipped in | dry ice |
| SMILES string | [P](=O)(OC2C(OC(C2)N)CO[P |
| storage temp. | −20°C |
| Application: | Plasmid DNA from Escherichia coli RRI has been used in polymerase chain reaction (PCR). |
| Application: | Plasmid DNA from E. coli RRI has also been used for in vitro DNA transfection studies. |
| Biochem/physiol Actions: | Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium. |
| Biochem/physiol Actions: | These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene; pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen. Foreign DNA inserted at the MCS abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium. The orientations of the MCS regions in the pUC plasmids are analogous to those of the corresponding M13 phage. |
| General description: | Plasmid DNA from Escherichia coli RRI confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene. pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen. |
| Other Notes: | pUC18 Plasmid DNA, 10 μg is supplied at approximately 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41105600 |