REDTaq® DNA Polymerase
SIGMA/D4309 - Taq for routine PCR with inert dye, 10X buffer included
Synonym: DNA polymerase with loading dye; Taq DNA polymerase
MDL Number: MFCD01635818
Product Type: Chemical
No loading buffers or tracking dyes required with Sigma REDTaq DNA Polymerase.
Samples may be added directly to an agarose gel after PCR without the addition of a loading buffer or tracking dye. The dye in REDTaq acts as a tracking dye migrating at approximately the same rate as a 125 bp fragment.
Samples may be added directly to an agarose gel after PCR without the addition of a loading buffer or tracking dye. The dye in REDTaq acts as a tracking dye migrating at approximately the same rate as a 125 bp fragment.
Same great performance as standard Taq.
Comparison of yield for 1, 2, 3, and 7 kb DNA fragments using REDTaq (R) and standard Taq (T) DNA polymerases under identical PCR cycling conditions.
Comparison of yield for 1, 2, 3, and 7 kb DNA fragments using REDTaq (R) and standard Taq (T) DNA polymerases under identical PCR cycling conditions.
REDTaq allows quick recognition and confirms appropriate mixing.
Vial 1 contains no REDTaq, vial 2 has 2.5 units of REDTaq added to a 50 μL reaction volume, and vial 3 shows a REDTaq PCR reaction solution thoroughly mixed.
Vial 1 contains no REDTaq, vial 2 has 2.5 units of REDTaq added to a 50 μL reaction volume, and vial 3 shows a REDTaq PCR reaction solution thoroughly mixed.
Representative kit contents
| application(s) | agriculture |
| color | red |
| concentration | 1 unit/μL |
| feature | dNTPs included: no |
| hotstart: no | |
| form | liquid |
| input | purified DNA |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| suitability | suitable for PCR |
| technique(s) | PCR: suitable |
| usage | sufficient for 1000 reactions |
| sufficient for 250 reactions | |
| sufficient for 50 reactions |
| Application: | For routine PCR amplifications REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis. |
| Features and Benefits: | • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications. • Visual confirmation that not only has the enzyme been added, but that proper mixing has occurred. • No additional loading dyes are necessary. An aliquot can be taken directly from the reaction and loaded onto an agarose gel for electrophoresis. |
| General description: | REDTaq® DNA Polymerase is a unique blend of Sigma′s quality Taq DNA Polymerase combined with an inert red dye. This dye enables quick visual confirmation of enzyme addition and reaction mixing. An aliquot of the samples (5-10 μl) can then be loaded directly onto an agarose gel for electrophoresis following PCR. The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 base pair fragment in a 1% agarose gel. Since no additional loading buffers are added to the reaction following PCR, reamplification is possible. The red dye has no effect on automated or manual sequencing, restriction digestions or other downstream applications. However, if removing the dye is desired, this can easily be accomplished using any standard purification method. |
| Legal Information: | REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims. |
| Other Notes: | One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C. |
| Packaging: | Provided with 10X reaction buffer containing MgCl2 |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352204 |