Anti-Derlin-1 antibody produced in rabbit
SIGMA/D4443 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-DERtrin 1; Anti-Degradation in endoplasmic reticulum protein 1; Anti-Derl-like protein 1
MDL Number: MFCD08705450
Product Type: Chemical
Western Blotting JOURNAL CITATION: Molecular characterization and expression of DERL1 in bovine ovarian follicles and corpora lutea. By: Ndiaye, K., Lussier, J. G., et al. in Reprod Biol Endocrinol, 2010. PubMed ID: 20682045 Image collected and cropped by CiteAb from the following publication, (http://rbej.biomedcentral.com/articles/10.1186/1477-7827-8-94), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Molecular characterization and expression of DERL1 in bovine ovarian follicles and corpora lutea. By: Ndiaye, K., Lussier, J. G., et al. in Reprod Biol Endocrinol, 2010. PubMed ID: 20682045 Image collected and cropped by CiteAb from the following publication, (http://rbej.biomedcentral.com/articles/10.1186/1477-7827-8-94), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Indirect Immunofluorescence Rat NRK cells (in the left panel) and human HeLa cells (in the right panel) were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton-X-100. Fixed cells were stained with 5 μg/mL Anti-Derlin antibody (Cat. No. D4443). The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.
Western Blotting Whole extract of HeLa cells were separated on SDS-PAGE and probed with Anti-Derlin-1 antibody produced in Rabbit (Cat. No. D4443). The antibody was developed with 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.2 μg/mL antibody 2. 0.4 μg/mL antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~22 kDa |
| shipped in | dry ice |
| species reactivity | rat, hamster, monkey, mouse, bovine, human, canine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2.5-5 μg/mL using rat NRK cells |
| western blot (chemiluminescent): 0.2-0.4 μg/mL using whole extract of human HeLa and mouse 3T3 cells. | |
| UniProt accession no. | Q9BUN8   |
| Application: | Anti-Derlin-1 antibody produced in rabbit has been used in: • immunostaining • co-immunoprecipitation • immunofluorescence |
| Biochem/physiol Actions: | Derlin-1 can interact with peptide:N-glycanase (PNGase), a deglycosylating enzyme, bringing it close to misfolding dislocating glycoproteins. |
| Biochem/physiol Actions: | Derlin-1 is required for the dislocation of misfolded proteins from the ER lumen to the cytosol, where they are destroyed by the ubiquitin-proteasome system. It interacts with PNGase, a deglycosylating enzyme, bringing it close to misfolding dislocating glycoproteins. It forms a membrane protein complex with VIMP ( (VCP-interacting membrane protein) and this complex serves as a receptor for p97. p97 interacts with several ubiquitin ligases, thus recruiting them to Derlin-1. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Derlin-1 is a 22kDa hydrophobic protein that spans the lipid bilayer of the ER four times with its amino- and carboxy-terminus in the cytosol. It is expressed with high levels in liver, spleen, pancreas, lung, thymus, and ovary. |
| General description: | Derlin-1 shares human homology with yeast Der1p. |
| Immunogen: | a synthetic peptide corresponding to the C-terminal region of human Derlin-1 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |