Deoxyribonuclease I from bovine pancreas
SIGMA/D4513 - Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein
Synonym: DNase I; Deoxyribonucleate 5′-oligonucleotido-hydrolase
CAS Number: 9003-98-9
EC Number: 232-667-0
MDL Number: MFCD00130918
Product Type: Chemical
| application(s) | diagnostic assay manufacturing diagnostic assay manufacturing |
| biological source | bovine pancreas |
| composition | Protein, ≥80% |
| foreign activity | Chymotrypsin ≤0.01% |
| Protease ≤0.005% | |
| RNase ≤0.01% | |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | ~31 kDa |
| packaging | vial of ≥10.0 mg protein |
| purified by | chromatography |
| Quality Level | 300  |
| solubility | 0.15 M NaCl: soluble 5.0 mg/mL, clear(lit.) |
| specific activity | ≥2,000 units/mg protein |
| sterility | sterile-filtered |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | DNA purification: suitable |
| type | Type II-S |
| Analysis Note: | Protein determined by biuret. |
| Application: | Deoxyribonuclease I from bovine pancreas has been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the isolation and further characterization of carp liver DNase. |
| Application: | DNAse I from Sigma has been compared with human urine-derived interleukin 1 inhibitor for the ability to hydrolyze [14C]DNA [14C]DNA. It has also been used to cleave a 139 base pair Hind III/Nci I restriction fragment to investigate the stability of the enzyme for use in footprinting experiments. DNase I is widely used as a footprinting agent for studying drug and protein binding to DNA. |
| Application: | Used for the removal of DNA from protein samples. |
| Biochem/physiol Actions: | DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum lies between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity. |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research  |
| Physical form: | Lyophilized powder containing calcium chloride |
| Preparation Note: | The enzyme powder may be reconstituted in water or any buffer at pH 4.0-9.0, except phosphate buffer. Calcium chelators should be avoided. 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration. |
| Unit Definition: | One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 5.0 at 25 °C, using DNA, Type I or III as substrate. (1 unit = 1 Kunitz unit) |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.21.1 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |