Deoxyribonuclease I from bovine pancreas
SIGMA/D4527 - Type II, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein
Synonym: DNase I; Deoxyribonucleate 5′-oligonucleotido-hydrolase
CAS Number: 9003-98-9
EC Number: 232-667-0
MDL Number: MFCD00130918
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D4527-200KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D4527-500KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D4527-10KU
| application(s) | diagnostic assay manufacturing diagnostic assay manufacturing |
| biological source | bovine pancreas |
| composition | Protein, ≥80% |
| foreign activity | Chymotrypsin ≤0.01% |
| Protease ≤0.005% | |
| RNase ≤0.01% | |
| form | lyophilized powder |
| mol wt | ~31 kDa |
| purified by | chromatography |
| Quality Level | 300  |
| shipped in | wet ice |
| solubility | 0.15 M NaCl: soluble 5.0 mg/mL, clear |
| specific activity | ≥2,000 units/mg protein |
| storage temp. | −20°C |
| suitability | suitable for molecular biology |
| technique(s) | DNA extraction: suitable |
| type | Type II |
| Analysis Note: | Protein determined by biuret. |
| Application: | Deoxyribonuclease I from bovine pancreas has been used for the identification, localization and expression of two novel human genes similar to deoxyribonuclease I. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate a new approach to obtaining high-activity RNase, DNase, cholesterolesterase, and trypsin from cattle pancreas. |
| Application: | DNAse I is used to nick DNA, as a first step to incorporate labeled bases into DNA. The enzyme from Sigma has been used in the nuclease stock along with RNAse during cell lysate preparation of Madin-Darby canine kidney (MDCK) II cell lines. It has also been used during RNA extraction from Sindbis virus. |
| Application: | Used for the removal of DNA from protein samples. |
| Biochem/physiol Actions: | DNase I is an endonuclease that acts on phosphodiester bonds adjacent to pyrimidines to produce polynucleotides with terminal 5′-phosphates. In the presence of Mg2+, DNAse I cleaves each strand of DNA independently and the cleavage sites are random. Both DNA strands are cleaved at approximately the same site in the presence of Mn2+. The pH optimum is found to be between 7 and 8. Divalent cations such as Mn2+, Ca2+, Co2+, and Zn2+ are activators of the enzyme. A concentration of 5 mM Ca2+ stabilizes the enzyme against proteolytic digestion. DNAse I from bovine pancreas consists of four chromatographically distinguishable components, A, B, C, and D, with their molar ratios being 4:1:1 respectively. Only minor amounts of D are found. 2-Mercaptoethanol, chelators, sodium dodecyl sulfate (SDS) and actin are known to inhibit the enzyme activity. |
| Features and Benefits: | Effective for nicking DNA and removal of DNA from protein samples |
| Other Notes: | One Kunitz unit will produce a change in A260 of 0.001 per minute per ml at pH 5.0 at 25 °C using DNA, Type I or III, as the substrate. |
| Packaging: | 10000 units in glass bottle |
| Packaging: | 20000, 40000, 200000, 500000 units in poly bottle |
| Physical form: | Contains calcium chloride |
| Preparation Note: | The enzyme powder may be reconstituted in water or any buffer at pH 4.0-9.0, except phosphate buffer. Calcium chelators should be avoided. 10 mg/mL solution of DNAse I in 0.15 M NaCl may lose <10% of its activity when stored for a week in aliquots at −20 °C. The same solutions stored in aliquots at 2-8 °C can lose approximately 20% activity. It remains active for up to five hours at 60 °C between pH 5 and 7, and loses activity in <10 minutes at 68 °C. It loses activity at the rate of 6%/hour in acetate buffer (pH 5.0) and tris buffer ((pH 7.2) at 1 mg/mL concentration. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥2,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.21.1 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |