Taq DNA Polymerase from Thermus aquaticus
SIGMA/D4545 - with 10× PCR reaction buffer without MgCl2
Synonym: Taq polymerase; Taq polymerase enzyme
CAS Number: 9012-90-2
MDL Number: MFCD00166026
Product Type: Chemical
Representative kit contents
| application(s) | agriculture |
| biological source | enzyme from bacterial (Thermus Aquaticus) |
| color | colorless |
| concentration | 5 units/μL |
| feature | dNTPs included: no |
| hotstart: no | |
| form | liquid |
| input | purified DNA |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| suitability | suitable for PCR and automated sequencing reactions |
| technique(s) | PCR: suitable |
| usage | sufficient for 1500 reactions |
| sufficient for 250 reactions | |
| sufficient for 50 reactions | |
| sufficient for 5000 reactions |
| Application: | Taq DNA Polymerase from Thermus aquaticus has been used: • in the process of DNA extraction (during gene amplification and sequencing) • in genotyping • in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8) • for amplification of RNA from primary endothelial cells by conventional PCR |
| Biochem/physiol Actions: | Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities. |
| Features and Benefits: | • MgCl2 provided in a separate tube to allow MgCl2 optimization • Can withstand repeated heating to 95 °C without significant loss of activity |
| General description: | Taq polymerase is a thermostable DNA polymerase named after the thermophilic bacterium Thermus aquaticus. A stable deoxyribonucleic acid (DNA) polymerase (with a temperature optimum of 80°C) has been purified from the extreme thermophile Thermus aquaticus. The enzyme is free from phosphomonoesterase, phosphodiesterase and single-stranded exonuclease activities. Maximal activity of the enzyme needs all four deoxyribonucleotides and activated calf thymus DNA. |
| Legal Information: | Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims. |
| Other Notes: | One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C. |
| Other Notes: | Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity. |
| Packaging: | Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification. |
| Packaging: | Taq DNA Polymerase with 10× reaction buffer without MgCl2. Includes a separate tube of 25 mM MgCl2 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.7 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |