N,N-Dimethylformamide
SIGMA/D4551 - Molecular Biology, ≥99%
Synonym: DMF; NSC 5356
CAS Number: 68-12-2
Empirical Formula (Hill Notation): C3H7NO
Molecular Weight: 73.09
EC Number: 200-679-5
MDL Number: MFCD00003284
Linear Formula: HCON(CH3)2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: D4551-500ML.
| assay | ≥99% |
| autoignition temp. | 833 °F |
| bp | 153 °C (lit.) |
| density | 0.944 g/mL (lit.) |
| expl. lim. | 15.2 % |
| form | liquid |
| grade | Molecular Biology |
| impurities | ≤0.5% water water |
| InChI | 1S/C3H7NO/c1-4(2)3-5/h3H, |
| InChI key | ZMXDDKWLCZADIW-UHFFFAOYSA |
| mp | −61 °C (lit.) |
| Quality Level | 200  |
| refractive index | n |
| SMILES string | [H]C(=O)N(C)C |
| storage temp. | room temp |
| suitability | suitable for solvent for chromogenic substrates (X-Gal, etc.) used in molecular biology applications. |
| vapor density | 2.5 (vs air) |
| vapor pressure | 2.7 mmHg ( 20 °C) |
| Application: | N,N-Dimethylformamide was used to solubilize MTT crystals in cell viability assays. It was also used in feruloyl esterase activity assay in molds exhibiting high activity of the enzyme. |
| Application: | Solvent for many hydrophobic organic compounds. |
| General description: | N, N-Dimethylformamide (DMF) is a solvent with a low evaporation rate, useful for preparing solutions with a variety of hydrophobic organic compounds used in molecular biology applications. |
| Packaging: | 250, 500 mL in glass bottle |
| Symbol |    GHS02,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H312 + H332 - H319 - H360D |
| Precautionary statements | P210 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 61-20/21-36 |
| Safety Statements | 53-45 |
| RIDADR | UN 2265 3 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | 135.5 °F - closed cup |
| Flash Point(C) | 57.5 °C - closed cup |
| Purity | ≥99% |
| bp | 153 °C (lit.) |
| mp | −61 °C (lit.) |
| Density | 0.944 g/mL (lit.) |
| Refractive Index | n |
| Storage Temp. | room temp |
| UNSPSC | 12352111 |