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Deoxyribonucleic acid from calf thymus

SIGMA/D4764 - Genomic, unsheared

Synonym: double-stranded template DNA; Calf Thymus DNA; DNA from calf thymus; Thymonucleic acid

CAS Number: 91080-16-9
EC Number: 293-507-3
MDL Number: MFCD00151698
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-D4764-.25UN 0.25 unit
 $49.90
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45-D4764-1UN 1 unit
 $127.00
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45-D4764-5UN 5 units
 $427.00
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biological source bovine (calf) thymus
form lyophilized powder
grade Molecular Biology
mol wt >20 kb
Quality Level 200 
storage temp. 2-8°C
Application: Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Application: Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.
Biochem/physiol Actions: 42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Features and Benefits: • No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
General description: High quality double-stranded template DNA isolated from the thymus of male and female calves.
Packaging: 0.25, 1 unit in glass bottle
Packaging: 5 units in glass bottle
Preparation Note: Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Preparation Note: Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 41106310

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