REDAccuTaq® LA DNA Polymerase
SIGMA/D4812 - High fidelity Taq with inert dye, 10X buffer included
MDL Number: MFCD01092095
Product Type: Chemical
REDTaq allows quick recognition and confirms appropriate mixing.
Vial 1 contains no REDTaq, vial 2 has 2.5 units of REDTaq added to a 50 μL reaction volume, and vial 3 shows a REDTaq PCR reaction solution thoroughly mixed.
Vial 1 contains no REDTaq, vial 2 has 2.5 units of REDTaq added to a 50 μL reaction volume, and vial 3 shows a REDTaq PCR reaction solution thoroughly mixed.
Longer products with improved fidelity with AccuTaq LA DNA polymerase Amplification of 2.5, 7, 9, 10, 12, and 15kb fragments of lambda DNA. 2.5 μg of DNA was amplified using 2.5 units of enzyme. The resulting products (6% of total reaction)were electrophoresed on a 0.8% agarose gel. Lanes 3-8 contained standard Taq DNA polymerase and Lanes 11-16 contained AccuTaq LA DNA polymerase. Lanes 1, 9. 1 kb DNA Marker 2, 10. Lambda Hind III DNA Marker 3, 11. 2.5 kb 4, 12. 7 kb
Representative packaging
| color | red |
| concentration | 1 unit/μL |
| feature | dNTPs included: no |
| hotstart: no | |
| Long & Accurate PCR | |
| form | liquid |
| input | purified DNA |
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | PCR: suitable |
| usage | sufficient for 250 reactions |
| Application: | REDAccuTaq® LA DNA Polymerase produce long DNA amplicons for: • Mutation analysis • Cloning genes • DNA sequencing • cDNA library generation • Amplicon sequencing |
| Features and Benefits: | • Increased fidelity, up to 6.5× that of Taq DNA polymerase • Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA • Visual confirmation that enzyme has been added, and proper mixing has occurred • No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis |
| General description: | REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods. |
| General description: | REDAccuTaq® long and accurate (LA) DNA Polymerase is a blend of Taq DNA polymerase and a small amount of thermostable proofreading polymerase that exhibits 3’→5’ exonuclease and an inert red dye that acts as a tracer and loading buffer. It is suitable for amplifying complex DNA templates like human genomic DNA. |
| Legal Information: | REDAccuTaq is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. |
| Other Notes: | One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C. |
| Other Notes: | Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 °C. |
| Packaging: | The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |