Deoxyribonucleic acid sodium salt from Escherichia coli strain B
SIGMA/D4889 - Genomic, unsheared
Synonym: DNA
CAS Number: 93384-16-8
EC Number: 297-226-7
MDL Number: MFCD00130922
Product Type: Chemical
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| biological source | Escherichia coli |
| form | lyophilized powder |
| grade | Molecular Biology |
| mol wt | >45 kb |
| Quality Level | 200  |
| solubility | water: ~1 g/L |
| storage temp. | 2-8°C |
| Analysis Note: | Analyzed by gel electrophoresis and visualization after ethidium bromide staining, with single predominant band of 45 kB or greater. |
| Application: | The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage λ, and in heterologous pre-hybridization and hybridization protocols. Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for: • PCR reactions of genomic DNA of Gardnerella vaginalis • nick translation and radiolabeling of dNTP • generating standard curve for 16S rDNA quantification • transfecting neutrophils • testing the extraction methods |
| Biochem/physiol Actions: | 50% GC; Tm = 90.5 °C |
| General description: | Escherichia coli strain B is most widely studied DNA after k-12. The genomes of E coli strain B and k-12 show differences in the IS sequence elements. Strain B have B-islands and express O-specific side-chain LPS. The product is unsheared genomic DNA. |
| Other Notes: | DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use. |
| Other Notes: | One unit will yield an A260 of 1.0 in 1.0 ml of 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA (1 cm light path). |
| Preparation Note: | Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106310 |