Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal
SIGMA/D4941 - clone DKPS308, purified from hybridoma cell culture
Synonym: Anti-DAPK; Anti-ROCO3
MDL Number: MFCD04040944
Product Type: Chemical
Immunoblotting Whole extract of HEK-293 cells overexpressing human DAP Kinase was separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-phospho-DAP-Kinase (pSer308) Clone: DKPS308 (Cat. No. D4941). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. HEK-293 cells overexpressing human DAP-Kinase (WT) 2. HEK-293 cells overexpressing human DAP-Kinase (mutant S308A)
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | DKPS308, monoclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~160 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer308) |
| technique(s) | immunoprecipitation (IP): suitable |
| indirect ELISA: suitable | |
| microarray: suitable | |
| western blot: 1-2 μg/mL using 293T (human embryonal kidney) cells transfected with DAP-kinase expression vector. | |
| UniProt accession no. | P53355  |
| Application: | Anti-phospho-DAP-Kinase (pSer308) antibody, Mouse monoclonal has been used in: • western blot assay • immunocytochemistry • enzyme linked immunosorbent assay (ELISA) • immunoprecipitation |
| Application: | Monoclonal anti-phospho-DAP-kinase (pSer308) antibody can be used in indirect ELISA, immunoblotting and immunoprecipitation. It can also be used in western blotting. |
| Biochem/physiol Actions: | Death Associated Protein Kinase (DAPK) plays crucial role in programmed cell death as well as in autophagy. DAPK activity is regulated by phosphorylation. Autophosphorylation at Ser308 on the calmodulin regulatory domain negatively regulate DAPK activity. This autophosphorylation, which occurs in cells at the basal state, lowers the affinity of DAPK for calmodulin and thus the kinase is inactive. Under some apoptotic conditions DAPK undergoes dephosphorylation. Consequently, it binds to calmodulin with higher affinity, becomes activated, phosphorylates its downstream substrate proteins, and mediates apoptosis. Monoclonal anti-phospho-DAP-kinase (pSer308) antibody can be used to study the mechanism of DAPK activation in apoptosis. It can also be used in microarray. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti-phospho DAP-Kinase (pSer308) (mouse IgG1 isotype) is derived from the DKPS308 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a phosphopeptide of human DAP-kinase, conjugated to KLH. Death Associated Protein Kinase (DAPK) is characterized with a multidomain structure including subdomain typical of serine/threonine kinases, a Ca2+/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. In addition, it also contains two auto-inhibitory domains, one of them Ca2+/calmodulin dependent. In the absence of this latter domain, DAPK is constitutively active. |
| Immunogen: | Phosphopeptide corresponding to amino acids 303-312 (pSer308) of human DAP-kinase, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |