Dipeptidyl Peptidase IV human
SIGMA/D4943 - recombinant, expressed in baculovirus infected Sf9 cells, pkg of ≥1.0 units/vial, ≥10 units/mg protein
Synonym: CD26; DPPIV; Dipeptidyl aminopeptidase IV; Glycoprotein GP110
MDL Number: MFCD00166456
Product Type: Chemical
Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. Both the human and porcine kidney enzymes exist as homodimers with a subunit molecular mass of ~30 kDa. Human recombinant DPPIV (Cat. No. D4943) is supplied as the 105 kDa soluble form representing amino acid residues 29-766 with a C-terminal histidine tag.
| form | solution |
| mol wt | 105 kDa |
| packaging | pkg of ≥1.0 units/vial |
| Quality Level | 200  |
| recombinant | expressed in baculovirus infected Sf9 cells |
| shipped in | wet ice |
| specific activity | ≥10 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P27487  |
| Application: | Human dipeptidyl peptidase IV has been used to study interactive hemodynamic effects of its inhibition and angiotensin-converting enzyme inhibition in humans. Human dipeptidyl peptidase IV has also been used in a study that informed the understanding of Hymenoptera venom allergies. |
| Application: | The enzyme from Sigma has been used to study the LC-MS (liquid chromatography-mass spectrometry) based assay method for DPP-IV inhibitor screening and substrate discovery. |
| Biochem/physiol Actions: | DPPIV has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes N-terminal dipeptides from the unsubstituted N-terminus of peptides with the sequence of X-Pro-Z and X-Ala-Z. The optimum pH is found to be 7.4-8.7. DPPIV is involved in the regulation of several important physiological processes such as immune functions, inflammation, CNS, endocrine functions, bone marrow mobilization, cancer growth, cell adhesion, glucose hemostasis and sepsis/severe infection. |
| Biochem/physiol Actions: | DPPIV has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes N-terminal dipeptides from the unsubstituted N-terminus of peptides with the sequence of X-Pro-Z and X-Ala-Z. Where X is a nonspecific residue at the N terminus and Z cannot be proline or hydroxyproline. |
| Biochem/physiol Actions: | Native DPPIV is a ubiquitous type II transmembrane glycoprotein and a serine protease of the S9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. There is an 88% sequence homology between the human and porcine kidney enzymes. Both exist as homodimers with a subunit molecular weight of ~30 kDa. The high mannose 100 kDa DPPIV precursor is processed in the Golgi to yield a 124 kDa heavily N-and O-linked mature glycoprotein. It is then sorted to the apical membrane through the concerted action of both N- and O-linked glycans and its association with lipid microdomains. The porcine enzyme contains 18.3% carbohydrates, which the glycan composition is 0.9% fucose, 3.4% mannose, 5.1% galactose, 8.2% glucosamine, and 0.7% sialic acid. DPPIV is highly expressed on endothelial cells, epithelial cells, and lymphocytes. It is also present in plasma in its soluble form. |
| General description: | C-terminal histidine-tagged. Soluble form (residues 29-766) MW 105 kDa |
| Other Notes: | One unit will produce 1.0 μmole of p-nitroaniline from Gly- |
| Other Notes: | View more information on Dipeptidyl Peptidase IV at www.sigma-aldrich.com/enz. |
| Physical form: | Supplied as a solution in 10 mM Tris-HCl, pH 7.6, 200 mM NaCl, 1 mM EDTA and 10% glycerol. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥10 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.4.14.5 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |